# Recommended PhiX Loading for a NextSeq 550 Validation Run

A PhiX validation run confirms proper hardware and software performance of the instrument.

* Load 1.5pM PhiX for High-Output verification runs
* Load 1.5pM PhiX for Mid-Output verification runs

PhiX validation runs can be set up in manual mode or Local Run Manager

* For manual mode see [How to set up a PhiX validation run on the NextSeq 500/550 in manual mode](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001532.md)
* For Local Run Manager mode see [How to set up a PhiX validation run with Local Run Manager](/instrumentation/general/instrumentation-general-reference_material-list/000001318.md)

For loading concentrations on other platforms see [PhiX loading concentrations for validation runs on Illumina sequencing platforms](/instrumentation/general/instrumentation-general-reference_material-list/000001536.md)

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