Recommended PhiX Loading for a NextSeq 550 Validation Run

A PhiX validation run confirms proper hardware and software performance of the instrument.

• Load 1.5pM PhiX for High-Output verification runs

• Load 1.5pM PhiX for Mid-Output verification runs

PhiX validation runs can be set up in manual mode or Local Run Manager

• For manual mode see How to set up a PhiX validation run on the NextSeq 500/550 in manual mode

• For Local Run Manager mode see How to set up a PhiX validation run with Local Run Manager

For loading concentrations on other platforms see PhiX loading concentrations for validation runs on Illumina sequencing platforms

For any feedback or questions regarding this article (Illumina Knowledge Article #2068), contact Illumina Technical Support [e-mail].