Recommended PhiX Loading for a NextSeq 550 Validation Run

A PhiX validation run confirms proper hardware and software performance of the instrument.

• Load 1.5pM PhiX for High-Output verification runs

• Load 1.5pM PhiX for Mid-Output verification runs

PhiX validation runs can be set up in manual mode or Local Run Manager

• For manual mode see How to set up a PhiX validation run on the NextSeq 500/550 in manual mode

• For Local Run Manager mode see How to set up a PhiX validation run with Local Run Manager

For loading concentrations on other platforms see PhiX loading concentrations for validation runs on Illumina sequencing platforms

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