# Recommended PhiX Loading for a NextSeq 550 Validation Run A PhiX validation run confirms proper hardware and software performance of the instrument. * Load 1.5pM PhiX for High-Output verification runs * Load 1.5pM PhiX for Mid-Output verification runs PhiX validation runs can be set up in manual mode or Local Run Manager * For manual mode see [How to set up a PhiX validation run on the NextSeq 500/550 in manual mode](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-reference_material-list/000001532) * For Local Run Manager mode see [How to set up a PhiX validation run with Local Run Manager](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-reference_material-list/000001318) For loading concentrations on other platforms see [PhiX loading concentrations for validation runs on Illumina sequencing platforms](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-reference_material-list/000001536) \ \ \
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