Troubleshooting frozen MiSeq Control Software

Background

The MiSeq Control Software (MCS) may freeze if the instrument has not been power cycled or has been left idle for an extended period

Troubleshooting

1. If the system seems frozen during a run, listen to determine whether any of the MiSeq motors are being used for sequencing. This manifests as clicking or whirring sounds.

2. If noises can be heard, it is most likely that the run is still proceeding.

   • Let the run continue.

3. If there are no noises coming from the MiSeq after several minutes, try one of the following options:

   1. If the MCS does not close by selecting X in the upper right corner, **press "**CTRL+ALT+DEL" to open Task Manager. Right-click MiSeq Control Software and End Process.

   2. Perform a power cycle on the instrument following the steps in How to Power Cycle the MiSeq or How To Power Cycle the MiSeq Video.

4. If the MCS still does not close, perform a hard reboot by turning off the system by the power switch on the back, right side of the instrument.

   • NOTE: This is the least desirable option and should only be used if the MCS will not close using the previously described methods.

   • Leave the system off for about 5 minutes, then toggle the power switch to turn it back on.

   • If the system fails to boot or MCS fails to initialize after a hard reboot, perform an additional power cycle.

5. If after the above steps there are still issues with system reboot or MCS initialization, contact Illumina Technical Support for further assistance.

For any feedback or questions regarding this article (Illumina Knowledge Article #1890), contact Illumina Technical Support [e-mail].