# Updating the DRAGEN Microbial Enrichment (DME) Databases on the MiSeq i100 Series
**Background**
The MiSeq i100 Series platform allows on-instrument DRAGEN analysis using a variety of DRAGEN Applications, including the DRAGEN Microbial Enrichment Plus (DME+) application. This Application requires specific databases are installed on the instrument through the Applications user interface, and must be installed manually after updating the DME+ Application.
See the following instructions for updating the DME+ Application and installing DME+ Database files on MiSeq i100 Series instruments.
**Resolution Actions**
*Pre-Update Activities*
1. Ensure that the **Illumina Software Update Manager (ISUM) v2.0** and **MiSeq i100 Series Digital Package v1.1** are installed.
1. See instructions in the [MiSeq i100 Series Software v1.1.0 Release Notes & Update Instructions](https://support.illumina.com/downloads/miseq-i100-series-release-notes.html) for additional details if needed.
2. Navigate to the [MiSeq i100 Series Downloads](https://support.illumina.com/sequencing/sequencing_instruments/miseq-i100-plus/downloads.html) page, then download the following files under the **Workflow Installers** and **Microbial Database Installers** headers:
1. DRAGEN Microbial Enrichment Plus (DME+) v1.1.7 Application .iapp file (Figure 1).
2. DRAGEN Microbial Enrichment Plus (DME+) database .ires files as needed (Figure 2).
3. Transfer the **.iapp** and **.ires** files to an exFAT-formatted USB storage device or network-attached storage.
**Figure 1**: The DRAGEN Microbial Enrichment Plus .iapp Installation File.
**Figure 2**: The DRAGEN Microbial Enrichment Database Installers .ires files for the DRAGEN Microbial Enrichment Plus (DME+) application.
*Uninstall the Existing DME+ Database Files*
1. Login to the MiSeq i100 Series Control Software as a local **Administrator** user.
2. Select the **menu icon** in the top-left corner, then select **Settings**, then **Applications**.
3. Select the **DME+ v1.0.13 Application**.
4. On the **Configuration** page under the **Analysis** header, select the **trash can icon** to delete the Database.
5. Repeat **Step 4** for each currently installed Database.
*Uninstall the DME+ v1.0.13 Application*
1. Login to the MiSeq i100 Series Control Software as a local **Administrator** user.
2. Select the **menu icon** in the top-left corner, then select **Settings**, then **Applications**.
3. Select the **DME+ v1.0.13 Application**.
4. Select **Uninstall**, then follow the prompts to complete uninstallation of the **DME+ v1.0.13 Application**.
*Install the DME+ v1.1.7 Application*
Before performing the following steps, mount the exFAT-formatted USB storage device or network-attached storage under **External Storage**. See the [MiSeq i100 Series Documentation - External Storage](https://support-docs.illumina.com/IN/MiSeqi100Series/Content/IN/MiSeqi100/SysOutFolder.htm) for additional details.
1. Login to the MiSeq i100 Series Control Software as a local **Administrator** user.
2. Select the **menu icon** in the top-left corner, then select **Settings**, then **Applications**.
3. Select **Install**.
4. Navigate to the **DME+ v1.1.7 Application .iapp** file, then select **Open**.
5. The Control Software will then upload the file and display the **Application details** on completion.
6. Select **Install** and allow the Control Software to install the **DME+ v1.1.7 Application**.
*Install the DME+ Databases*
1. Login to the MiSeq i100 Series Control Software as a local **Administrator** user.
2. Select the **menu icon** in the top-left corner, then select **Settings**, then **Applications**.
3. Select the **DME+ v1.1.7 Application**.
4. On the **Configuration** page, select **Upload** file and then navigate to the **DME+ Database .ires file**, then select **Open**.
5. Follow the prompts to complete installation of the .ires Database file.
6. Repeat **Steps 4 and 5** for each Database file to be installed.
7. Select **Save**.
Once the Application update and associated Databases have been installed, **eject the USB storage device** or **remove the network-attached storage** under **External Storage** if no longer required. See the [MiSeq i100 Series Documentation - External Storage](https://support-docs.illumina.com/IN/MiSeqi100Series/Content/IN/MiSeqi100/SysOutFolder.htm) for additional details.
\
\
\
| |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #9962), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009962%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
---
# Agent Instructions: Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.
Perform an HTTP GET request on the current page URL with the `ask` query parameter:
```
GET https://knowledge.illumina.com/instrumentation/miseq-i100-series/instrumentation-miseq-i100-series-troubleshooting-list/000009962.md?ask=
```
The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.
Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.