Updating the DRAGEN Microbial Enrichment (DME) Databases on the MiSeq i100 Series

Background

The MiSeq i100 Series platform allows on-instrument DRAGEN analysis using a variety of DRAGEN Applications, including the DRAGEN Microbial Enrichment Plus (DME+) application. This Application requires specific databases are installed on the instrument through the Applications user interface, and must be installed manually after updating the DME+ Application.

See the following instructions for updating the DME+ Application and installing DME+ Database files on MiSeq i100 Series instruments.

Resolution Actions

Pre-Update Activities

1. Ensure that the Illumina Software Update Manager (ISUM) v2.0 and MiSeq i100 Series Digital Package v1.1 are installed.

   1. See instructions in the MiSeq i100 Series Software v1.1.0 Release Notes & Update Instructions for additional details if needed.

2. Navigate to the MiSeq i100 Series Downloads page, then download the following files under the Workflow Installers and Microbial Database Installers headers:

   1. DRAGEN Microbial Enrichment Plus (DME+) v1.1.7 Application .iapp file

   2. DRAGEN Microbial Enrichment Plus (DME+) database .ires files as needed.

3. Transfer the .iapp and .ires files to an exFAT-formatted USB storage device or network-attached storage.

Uninstall the Existing DME+ Database Files

1. Login to the MiSeq i100 Series Control Software as a local Administrator user.

2. Select the menu icon in the top-left corner, then select Settings, then Applications.

3. Select the DME+ v1.0.13 Application.

4. On the Configuration page under the Analysis header, select the trash can icon to delete the Database.

5. Repeat Step 4 for each currently installed Database.

Uninstall the DME+ v1.0.13 Application

1. Login to the MiSeq i100 Series Control Software as a local Administrator user.

2. Select the menu icon in the top-left corner, then select Settings, then Applications.

3. Select the DME+ v1.0.13 Application.

4. Select Uninstall, then follow the prompts to complete uninstallation of the DME+ v1.0.13 Application.

Install the DME+ v1.1.7 Application

Before performing the following steps, mount the exFAT-formatted USB storage device or network-attached storage under External Storage. See the MiSeq i100 Series Documentation - External Storage for additional details.

1. Login to the MiSeq i100 Series Control Software as a local Administrator user.

2. Select the menu icon in the top-left corner, then select Settings, then Applications.

3. Select Install.

4. Navigate to the DME+ v1.1.7 Application .iapp file, then select Open.

5. The Control Software will then upload the file and display the Application details on completion.

6. Select Install and allow the Control Software to install the DME+ v1.1.7 Application.

Install the DME+ Databases

1. Login to the MiSeq i100 Series Control Software as a local Administrator user.

2. Select the menu icon in the top-left corner, then select Settings, then Applications.

3. Select the DME+ v1.1.7 Application.

4. On the Configuration page, select Upload file and then navigate to the DME+ Database .ires file, then select Open.

5. Follow the prompts to complete installation of the .ires Database file.

6. Repeat Steps 4 and 5 for each Database file to be installed.

7. Select Save.

Once the Application update and associated Databases have been installed, eject the USB storage device or remove the network-attached storage under External Storage if no longer required. See the MiSeq i100 Series Documentation - External Storage for additional details.

For any feedback or questions regarding this article (Illumina Knowledge Article #9962), contact Illumina Technical Support [email protected].