How to Populate the Indexes Tab in Sequencing Analysis Viewer (SAV) for MiSeq i100 Runs

Background

On the MiSeq i100, the file containing the index demultiplexing metrics, named IndexMetricsOut.bin, is generated in the Demux folder within the Analysis folder at the following path:

//[server]/[share]/[output-directory]/[RunId]/Analysis/[#]/Data/Demux/IndexMetricsOut.bin

Sequencing Analysis Viewer (SAV) only retrieves metrics data from the InterOp folder.

How to Populate the Indexes Tab

To visualize the index demultiplexing metrics in SAV, the IndexMetricsOut.bin file must be copied to the InterOp folder.

1. Navigate to the Demux folder within the run directory: //[server]/[share]/[output-directory]/[RunId]/Analysis/[#]/Data/Demux

2. Copy the IndexMetricsOut.bin file and paste it into the InterOp folder:

• //[server]/[share]/[output-directory]/[RunId]/InterOp

1. Reopen or refresh SAV. The index demultiplexing metrics should then appear in the Indexes tab.

For any feedback or questions regarding this article (Illumina Knowledge Article #10140), contact Illumina Technical Support [email protected].