# How to requeue a MiSeq i100 Series run locally (on the instrument)

A MiSeq i100 Series analysis can be requeued locally from Illumina Run Manager. The run cannot be requeued if a sequencing run is in progress or if the run was deleted from the instrument.

Follow the steps described below to requeue local analysis:

1. Navigate to the **Runs** menu in Illumina Run Manager and select the run to requeue from the **Completed** tab. ![](/files/3gidDwsaWhOumd3QXGoc)
2. Select **Requeue Analysis** under the Secondary Analysis section to start the requeued analysis. Choose to requeue the analysis from the run, or from a new sample sheet. A new sample sheet can be selected from a previously mounted external storage folder or USB drive. Both options require the selection of an output folder.
3. Enter a reason for the reanalysis in the textbox.

![](/files/mXYKHHHg3zMM7Yhb0ZhY)

**Note:** When requeuing analysis from the sample sheet, if the “Skip sample sheet validation” option is enabled it will allow users to use a sample sheet that contains more than 1536 samples.

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                         |
| :---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #10108), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000010108%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/instrumentation/miseq-i100-series/instrumentation-miseq-i100-series-reference_material-list/000010108.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
