How to requeue a MiSeq i100 Series run locally (on the instrument)

A MiSeqi100 Series analysis can be requeued locally from Illumina Run Manager. The run cannot be requeued if a sequencing run is in progress or if the run was deleted from the instrument.

Follow the steps below to requeue local analysis:

1. Navigate to the Runs menu in Illumina Run Manager and select the run to requeue from the Completed tab.

2. Select Requeue Analysis under the Secondary Analysis section to start the requeued analysis. Choose to requeue the analysis from the previous sample information, or from a new sample sheet. Both options require the selection of an output folder. Enter a reason for the reanalysis in the textbox.

Note: When requeuing analysis from sample sheet, the “Skip sample sheet validation” option is enabled and it will allow users to use a sample sheet that contains more than 1536 samples.

For any feedback or questions regarding this article (Illumina Knowledge Article #10108), contact Illumina Technical Support [email protected].