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Flow cell not detected error on the NextSeq 1000/2000

Background: When starting a run, the NextSeq 1000/2000 can present a "flow cell not detected" error despite a properly loaded flow cell.

To troubleshoot:

  1. Investigate the flow cell seating and cleanliness.

  • Retry by reseating the flow cell.

  1. Power cycle* the system.

  1. If issue persists, prepare the following information and contact Illumina Technical Support for further guidance.

When reaching out to Illumina Technical Support, provide the following:

  • Control Software Logs: /usr/local/illumina/logs/nextseq1k2k-control-software

  • Service Software Logs: /usr/local/illumina/logs/nextseq1k2k-service-software

For any feedback or questions regarding this article (Illumina Knowledge Article #6639), contact Illumina Technical Support techsupport@illumina.com.

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