Troubleshooting Lane Pump Errors on the MiSeq

Background

The following error messages can occur if the pump that pulls reagents through the MiSeq fluidics system does not respond to commands sent by the MiSeq Control Software.

This error may result from hardware or communication errors on the MiSeq. Potential hardware-related causes include pump, pump controller, or syringe failure. In some cases, this is a temporary situation that requires no action. Note that an error mentioning the “Cavro” pump is a different issue, see Knowledge Article Troubleshooting Cavro Pump Error on MiSeq for details.

Figure 1: Error in LanePump. Syringe pump valve failed to move: Valve overload. Command = '/1Q\r', reply = 'j'

Troubleshooting Actions

1. Power cycle the MiSeq instrument.

   1. See Knowledge Article How to shut down or power cycle the MiSeq for details.

2. If MiSeq Control Software initializes as expected, perform ONE of the following options:

• Option 1: Perform a System Check

  • See Knowledge Article How to Perform a System Check on the MiSeq for details.

  • If the system check passes, users may setup the next run on the instrument and continue normal operation.

• Option 2: Perform a maintenance wash and measure the waste output to ensure the fluidics system is operating normally. The maintenance wash is expected to output 51.75 ml +/- 10% wash volume waste.

  • See Knowledge Article Wash best practices for the MiSeq for details.

  • If the maintenance wash successfully completes and returns the appropriate amount of waste, users may set up the next run on the instrument and continue normal operation.

1. If the lane pump error is not resolved by power cycling or persists in the System Checks or Maintenance wash, contact Illumina Technical Support for further assistance.

For any feedback or questions regarding this article (Illumina Knowledge Article #1446), contact Illumina Technical Support [e-mail].