How to requeue a NextSeq 1000/2000 run locally (on the instrument)

An analysis can be requeued locally from the Process Management screen in the NextSeq 1000/2000 Control Software.

Requirements to requeue an analysis:

1. The System is NOT currently sequencing.

2. The original run folder has not been deleted from the instrument.

   1. The run cannot be requeued once it has been deleted from Disk Management.

3. The run is listed as successfully completed in the control software.

Follow the steps below to requeue local analysis:

1. Select the menu in the top left corner > Process Management

2. Identify the run to be requeued

3. Under the "Sample Sheet" select "Choose..." and navigate to desired Sample Sheet.

When requeuing analysis:

• A new v2 sample sheet can be used. Transfer the sample sheet on a USB drive or network drive accessible to the instrument, and browse to it.

• A new output directory can be selected. If outputting to an external hard drive results in an error, the only local location that is large enough to be used as an output folder is /usr/local/Illumina/Images.

• If a new output directory is not selected, the new output data will be written to a new subfolder in the original output directory. Example: OutputDirectory/2/Data

For any feedback or questions regarding this article (Illumina Knowledge Article #5816), contact Illumina Technical Support [e-mail].