> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-faq-list/000003631.md).

# What is the maximum Sample ID length?

When creating SampleSheets, the Sample ID field is used to identify samples, and to name the FASTQ files generated.

The Sample ID field can contain alphanumeric characters, hyphens and underscores, with maximum lengths depending on the software used for Sample Sheet creation and analysis:

-BaseSpace Run Planning: Maximum of 100 characters.

-Local Run Manager v3: Maximum of 40 characters\*.

-Local Run Manager v4: Maximum of 90 characters\*.

**\*NOTE:** Local Run Manager will not allow for hyphens and underscores to be at the end of the Sample ID, they need to be in the middle.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3631), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003631%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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