Illumina Knowledge

Illumina has announced the End-of-Life timeline for MiSeq RUO, MiniSeq, and iSeq 100 Systems, and associated consumables. New Service Contracts may only be purchased until September 30, 2025. For more information click here.

Illumina Knowledge is a repository of 1625 FAQs, troubleshooting articles and reference material for Illumina products and workflows, covering all Illumina Microarrays and every stage of Sequencing.

Use the navigation bar on the left of the page to find areas of interest and content types, use the search function at the top to query keywords.

What's New

• Analysis frequently asked questions for the Illumina miRNA prep kit (October 16, 2025)

• Setting up a sample sheet to send all reads to Undetermined in Miseq Reporter (October 16, 2025)

• Library quantification and QC for the Illumina miRNA prep kit (October 14, 2025)

• Sequencing frequently asked questions for the Illumina miRNA prep kit (October 14, 2025)

• PhiX Indexed Control (1000 Cycle) Product Information (October 7, 2025)

	More...

Announcements

• Check out our NEW resource for End-to-end sequencing and microarray training at Illumina Solutions Centers in the Americas. (May 31, 2024)

	More...

Cloud Status

• Click the link above to check on the status of Illumina cloud software products.